Cell Name

GES-1

Organism

Homo sapiens, human

Age

Tissue

Morphology

epithelial

Growth Properties

adherent

Descriptions

Biosafety Level

2.Culture Conditions and Handling

Complete Growth Medium

RPMI-1640 (150110)+10% FBS (164210-500)+1% P/S (180120)

Remove and discard culture medium. Briefly rinse the cell layer with DPBS

solution to remove all traces of serum that contains trypsin inhibitor.

Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells

under an inverted microscope until cell layer is dispersed (usually within 3

Subculturing

to 5 minutes). Cells that are difficult to detach may be placed at 37°C to

facilitate dispersal.

Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently

pipetting. Add appropriate aliquots of the cell suspension to new culture

vessels.

Subc*tion Ratio

1:2-1:4

Medium Renewal

every 2 to 3 days

Cryopreservation

Freeze medium: 50% basal medium+40% FBS+10% DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: Air, 95%; CO2, 5%

Temperature: 37℃

3.Special Features of the Cell Line

Tumorigenic

Effects

Receptor Expression

Antigen Expression

Gene Expression

Applications