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技術(shù)文章Article 首 頁(yè) > 技術(shù)文章 > 95-D 人高轉(zhuǎn)移肺癌細(xì)胞
95-D 人高轉(zhuǎn)移肺癌細(xì)胞
點(diǎn)擊次數(shù)：1154 更新時(shí)間：2017-01-17
 

95-D   人高轉(zhuǎn)移肺癌細(xì)胞

General Information:

Organism:

Homo sapiens, human

Tissue:

lung

Culture Properties:

adherent

Morphology:

epithelial

 

Culture Method:

Complete Growth Medium:

RPMI-1640 (Gibco 31800-022) + 10% FBS + Penicillin/

Streptomycin

Subculturing:

Volumes are given for a 25cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

  • Remove and discard culture medium.
  • Briefly rinse the cell layer with 0.25%(w/v) Trypsin-EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  • Add 1.0 to 2.0mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Discard Trypsin-EDTA solution.
  • Add 6.0 to 8.0mL of complete growth medium and aspirate cells by gently pipetting.
  • Add appropriate aliquots of the cell suspension to new culture vessels.
  • Incubate cultures at 37°C, 5% CO2.

Subc*tion Ratio:

A subc*tion ratio of 1:2 to 1:4 is recommended

Cryopreservation:

Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase
 
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