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人膀胱癌細(xì)胞

點(diǎn)擊次數(shù)：1258 更新時(shí)間：2017-01-17

5637 人膀胱癌細(xì)胞

Cat NO.: CL-0002

General Information:

Organism:	Homo sapiens, human
Tissue:	urinary bladder
Culture Properties:	adherent
Morphology:	epithelial

Culture Method:

Complete Growth Medium:	RPMI-1640 + 10% FBS + Penicillin/Streptomycin
Subculturing:	Volumes are given for a 25cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25%(w/v) Trypsin-EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Add 6.0 to 8.0mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C, 5% CO₂.
Subc*tion Ratio:	A subc*tion ratio of 1:4 to 1:8 is recommended
Cryopreservation:	Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase

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